|The Biology of the Goat Laboratory|
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|How to do your own fecal exams|
Using the microscope to examine a sample
Set the slide on the microscope stage. On some microscopes you will have to move the slide around with your fingers.
If your microscope has a mechanical stage, like the one shown at the left, you will use the mechanical adjustment knobs to move the slide right, left, forward, and back.
Mechanical stages are easier to use if you want to scan the whole slide which you will want to do if you want to estimate an egg count.
Start with the lowest power which is usually 10X. Move the stage with the course focus knob to just below the tip of the lens while watching from the side so you don't break the cover slip.
Then, while looking through the eyepiece, focus down until you can clearly see the level of the fluid between the cover slip and slide. This can take some practice. It helps to have the corner of the cover slip in view then focus down a little bit or find a bit of debris and focus on it.
You may need to adjust the amount of light coming through the sample by opening or closing the diaphragm under the stage. You will need more light at the higher power.
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